The effect of storage at different temperatures on cholinesterase activity in human serum.
نویسندگان
چکیده
The effect of storage on the catalytic concentration of cholinesterase and on the reference values of cholinesterase in human serum were studied. When serum was stored at room temperature (20 degrees C), at 4 degrees C and at - 20 degrees C (one year) there was no change in catalytic activity. Even after nine times freezing and thawing (over nine weeks) the catalytic activity was unaffected. The average reference value was significantly higher for males than for females (3.11 +/- 0.57 vs 2.50 +/- 0.43 kU/l).
منابع مشابه
Effect of Gallic Acid on Reactivation of Acetylcholinesterase and Butyrylcholinesterase Inhibited by Diazinon in Vitro and in Vivo
Background and purpose: Diazinon is an organophosphate insecticide that binds to the acetylcholinesterase enzyme after metabolization causing its inactivation. Galic acid is a polyphenolic compound with nucleophilic properties. The aim of this study was to investigate the effects of gallic acid on reactivation of acetylcholine and butyrylcholinesterase inhibited by diazinon in mice and human se...
متن کاملEvaluation of germination and some biochemical indices of faba bean (Vicia faba L.) seeds stored at different temperatures and moisture content
This study was performed to investigate the effect of storage conditions on seed germination performance of faba bean (cv. Shami). For this purpose, a three factors factorial experiment was conducted in laboratory of Seed Science and Technology, Faculty of Agriculture, Yasouj University, in 2013-2014, based on CRD design with five replications. The experimental factors were storage temperature...
متن کاملComparative Measurement of Serum Acetyl Cholinesterase Enzyme Using Three Different Methods
Introduction: IAChE (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of Ach. Material & Method: We investigated three methods for the determination of AChE in human blood serum, using three different assays. Michel methods are based on the measurement of hydrogen ion produced in pro...
متن کاملComparative evaluation of five different storage media and temperature effect on human periodontal ligament fibroblast viability
Introduction: Following dental avulsion, the immediate recommended treatment is tooth replantation to avoid adverse effects. Therefore, the tooth must be stored in a physiological storage medium to preserve the viability of the periodontal ligament fibroblast (PDLF) cells during transportation to dental office. The aim of this study was to determine the effect of several storage media in preser...
متن کاملSerum Paraoxonase 1 Activity in Patients with Organophosphate Poisoning: A Potential Indicator of Prognosis
Background: Human serum paraoxonase 1 (PON1) hydrolyzes organophosphate (OP) compounds and so significantly alters an individual’s susceptibility to the toxicity of these chemicals. The study was designed to assess the serum PON1 activity in a series of patients with OP poisoning. Methods: Suspected OP poisoning patients presented within 6 hours of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 23 5 شماره
صفحات -
تاریخ انتشار 1985